El programa se basa en intervenciones que han demostrado ser eficaces (enfoque cognitivo-conductual). To conclude, a SWOT analysis is made available of transgenesis in environmental components.Prevención, Depresión, Adolescentes, Bienestar ResumenĮste estudio consistió en describir el desarrollo y la evaluación del Programa Sonrisa cuyo principal objetivo fue la prevención de la depresión y la promoción del bienestar en adolescentes. Due to the impact of the techniques used by the second Green Revolution and the various complex interactions among different environmental components, this paper provides a panoramic description open to discussion and research in soil science and landscape ecology, leading the precise determination of the conditions generated in the natural and human environment. ![]() This paper aims to review the history, development, and applications of the second Green Revolution framed within the advantages and disadvantages of the environmental components that interact on the soil. ![]() The debate on this revolution is maintained, sharpening and raising many interests due to its transversal aspects and impact on social, scientific, economic, political, and ecological issues. The second Green Revolution was established as a strategy to help ensure food security, an increasingly growing population, and demanding of resources, to optimize crop productivity improving or introducing specific characteristics such as resistance to insects and herbicide tolerance to allow GMOs to be more tolerant of natural aggression. To genetically modified organisms (GMOs) developed from inter-species cross manipulation and genetic information of some species of interest. The product of the second Green Revolution corresponds The second Green Revolution is understood as the development of transgenic varieties, using tools such as genetic engineering and molecular biology applied to the DNA molecule. rhizogenes strains LBA 9402, A4 using streptomycin, kanamycin and ampicillin for selection. The reproducibility of the method was assessed among the A. The genetic transformation of the bacteria and the plant tissue was verified by PCR and by β-glucuronidase assays. After the bacterium was transformed, it was cultured in liquid culture medium and plated on solid medium to isolate colonies. The objective of this study was to develop a heat-shock protocol for the transformation of Agrobacterium rhizogenes without the spontaneous generation of antibiotic resistance, to increase its sensitivity and specificity to produce transgenic hairy roots. has been widely observed, making it difficult to select for the transformed bacteria. During the agrotransformation of plant cells, tissue, and organs, the acquisition of antibiotic resistance by Agrobacterium spp. Although this phenomenon is primarily observed in medicine, it also affects the success of scientific research when bacterial-based genetic transformation experiments are performed. Es interesante notar que en la línea mostrada en la figura 6 Bb se observó intensa coloración azul en toda la raíz, mientras que en la línea mostrada en la figura 6 Bc solo se observó coloración en algunasĪntibiotic resistance occurs when the use of antibiotics to treat bacterial infections or to select for transformed bacteria from in vitro cultures becomes ineffective. No obstante, después de seleccionar entre varias líneas transformadas con el gen l1, se logró obtener tejido suficiente para investigar la presencia del gen heterólogo mediante el ensayo de de β-glucuronidasa observándose la coloración típica en una de las dos líneas de raíces probadas en concordancia con el control positivo de raíces transformadas con pCAMBIA y en contraste con el control negativo de raíces no transformadas (Figura 6 B). rhizogenes silvestre, 15 no con el T-DNA de pCAMBIA que lleva el gen l1 clonado. Este resultado sugiere que estos tejidos habían sido transformado solo con el T-DNA de A. El ensayo de β-glucuronidasa, como se esperaba, resultó negativo en comparación con raíces transformadas con el vector pCAMBIA 1105.1 vacío (Figura 6 A). rhizogenes transformadas con la construcción pCAMHPV16L1 murieron cuando se pusieron en contacto con higromicina, que es el marcador de selección para raíces transformadas con el vector pCAMBIA 1105.1. La mayoría de las raíces que se indujeron por punción de hipocotilos de plántulas con la cepa de A. B, Ensayo de β –glucuronidasa y morfología microscópica de raíces de brócoli transformadas positivamente (b, c) con el gen de la proteína l1 observadas al microscopio óptico a 100X. A, Microfotografías 25X y 100X después del ensayo de β–glucuronidasa de raíces no transformadas (a) y transformadas negativamente con gen l1 del HPV (b), en comparación con raíces transformadas con el vector pCAMBIA (c).
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